Lipid Profile

A fasting specimen (no food or drink, except for water) for 12- 14 hours before blood is drawn is preferred. However, your doctor may request that you have your blood drawn when you are not fasting.

Vitamin B12 and Folate

A fasting specimen (no food or drink, except for water) for 12- 14 hours before blood is drawn is preferred. However, your doctor may request that you have your blood drawn when you are not fasting.

Digoxin Level

Blood should be drawn 6-8 hours after the last dose of digoxin was taken.

C-Peptide

Patient should be fasting (no food or drink, except for water) for 12-14 hours before blood is drawn.

Cortisol

Cortisol may be tested in blood or urine.

If blood cortisol levels are requested by your physician, you may be asked to have your blood drawn once (random cortisol) or multiple times. Your physician will inform you if you are having multiple blood draws for your cortisol testing.
Cortisol blood tests may be drawn at about 8 am, when cortisol should be at its peak, and again at about 4pm, when the level should have dropped. Sometimes a resting sample will be obtained late in the evening.

If your physician requests a urine cortisol, you will be asked to collect either a 24-hour urine or a single first morning specimen. Your physician will inform you of the type of urine collection you will need. Please obtain a collection container from Outpatient services or the laboratory if after hours. Be sure to contact your physician for specific instructions.

Sputum for Culture and Acid Fast Mycobacterium (Afb)

1. Patient should rinse mouth and gargle with water immediately prior to collection.
2. Collect specimen from deep cough into a sterile container.
3. Patient should avoid any contamination with saliva.
4. Return specimen as soon as possible. If there is a delay, specimen should be 
refrigerated. Please label the specimen
5. contaIner with patient’s name, date and time.

Semen Analysis

1. Refrain from sexual intercourse or masturbation for between 3 days before testing.
2. Produce the sample by masturbation without artificial lubricants. Do not use a 
condom, as condoms contain spermicidal agents.
3. Collect the sample into the clean, wide mouth container supplied. It is important that 
the whole ejaculate is collected. If not, the specimen should be labeled as incomplete.
4. Within 1 hour of collection, bring the sample to the 
laboratory. Keep the specimen warm in a pocket near your body.
5. Label the specimen with name, date, and time of the collection.
6. Complete the patient questionnaire (found in OP registration or Lab front desk) and 
return to Outpatient services or laboratory with the specimen.

Glucose Tolerance Test

Non-Pregnant

Patient should be fasting for 12 to 14 hours (no food or drink, except for water). A blood specimen will be drawn and tested. You will then be given a special drink. Your blood will be drawn once each hour after you finish the drink. The number of hours may vary from 2 to 6. Your doctor will decide how many hours you will be tested.

Please allow plenty of time for this test to be completed. You will need to allow approximately one hour more than the number of hours your physician requested. For example, if your physician ordered a 3 hour glucose tolerance, please allow 4 hours for the test to be completed.

Prenatal Glucose Tolerance Tests
For our pregnant patients:

Your physician may order glucose tolerance testing during your pregnancy.

50 Gram 1 Hour Glucose Tolerance Test (Gestational Diabetes Screen)

1. No special patient preparation is required.
2. This test is done without regard to the time of day or time of last meal. You do not 
need to fast before this test is given.
3. You will be given a special drink and your blood will be drawn one hour after you 
finish the drink.
4. Please allow at least 1 1⁄2 hours for this test to be completed.

100 Gram 3 Hour Glucose Tolerance Test (Gestational Diabetes)

1. This test should be performed in the morning after an overnight fast of at least 8 hours 
but not more than 14 hours and after at least 3 days of unrestricted diet and activity.
2. A blood specimen will be drawn and tested.
3. You will then be given a special drink.
4. Your blood will be drawn once each hour after you finish the drink for three hours.
5. Please allow plenty of time for this test. You will need to allow at least 4 hours for this test to be completed.

Stool Specimen Collections

24-Hour Urine Specimens

Please follow these instructions in collecting your 24-hour urine specimen.
You will find it more convenient to void (urinate) into the smaller container provided and transfer the urine into the larger collection container.
Do not add anything but urine to the container and do not pour out any liquid or powder that may already be in the collection container. These substances may cause burns if touched.
The collection container should be kept cool throughout the collection period. You may place in a refrigerator or in a pan with ice during collection.

1. Upon arising in the morning, urinate into the toilet, emptying your bladder completely. Do not collect this sample. Note the exact time and print it on the container label.
2. Collect all urine voided for 24 hours after this time in the container provided. All urine passed during the 24-hour time period (day and night) must be saved. Urine passed during bowel movements must also be collected. Be careful not to contaminate urine specimen with feces.
3. Refrigerate the collected urine between all voidings or keep in a cool place.
4. At exactly the same time the following morning, void completely again (first time 
after awakening) and add this sample to the collection container. This completes your 
24-hour collection.
5. Be sure to label specimen with patient’s name, date and time the collection began and 
ended.
6. Take the 24-hour specimen to the Outpatient service area as soon as possible.

CONTAINERS:
Sterile containers and 24 hr urine containers may be picked up at Outpatient service between the hours of 8am to 4pm.

24-Hour Creatinine Clearance Collection

1. Obtain a collection container from Outpatient services/ or laboratory if after hours.
2. Follow the directions for 24-hour urine collections.
3. Keep the collection container with urine refrigerated or on ice.
4. Have your blood drawn either at the beginning, during or end of the collection period. 
A blood sample is needed in conjunction with the 24-hour urine collection in order to complete the testing.
5. Please label the container with the patient’s name, date, and time the collection began and ended.
6. Include the patient’s height and weight.
7. Return patient collection container to laboratory.

24-Hour Urine –Total Protein Collection

1. Obtain a collection container from laboratory
2. Follow instructions for 24-hour urine collection.
3. Keep the collection container refrigerated or on ice.
4. Please label the container with patient’s name, date and time the collection began and 
ended.
5. Return patient collection container to laboratory.

Urine For Urinalysis And/Or Culture

Obtain a sterile container from the laboratory. Please avoid touching the inside of the container and/or the lid in order to maintain sterility.

1. Female: The patient should be seated on the toilet or bedpan with legs separated. Separate labia minor (inner folds), cleanse opening of urethra three times with cleansing pads. Allow the initial stream of urine to pass, and then collect urine into a sterile container.
2. Male: cleanse glans three times with cleaning pads. Allow the initial stream of urine to pass, and then collect urine into a sterile container.
3. Please label the specimen with patient’s name, date and time of collection.
4. The specimen should be returned as soon as possible. If there is a delay, the 
specimen should be refrigerated.

Urine Specimens

Stool For Occult Blood

Diet and drugs may affect results of occult blood testing. Please talk to your physician before making any changes in diet or medications prescribed for you.

1. 2. For three days before and during stool collection eat a well-balanced diet including fiber such as bran cereals, fruits and vegetables. Avoid red meats, including beef, lamb, and liver.

There are also some common over-the-counter drugs that may affect results of the testing. For seven days before and during the stool collection period, avoid non- steroidal anti-inflammatory drugs such as ibuprofen, naproxen, or aspirin. 
o Note: Aspirin may be taken if not in excess of one adult aspirin or 325mg per day. 
o Acetaminophen (Tylenol) may be taken as needed.

For three days before and during the stool collection period, avoid vitamin C in 
excess of 250mg/day from supplements, citrus fruits, and juices.

Iron supplements may be taken if they do not contain more than 250 mg of 
vitamin C. 
Your doctor or laboratory will give you one or more test “cards”.
You will collect a separate sample from three different stools, usually on consecutive 
days. Each stool specimen should be collected into a clean container and should not be contaminated with urine or water.

3. Each card should be labeled with your name and the date and time of collection.
4. Open the flap on the card.
5. With an applicator stick, apply a thin smear of stool onto each filter 
paper/square/window contained on the card.
6. Allow the filter paper to dry. Once it is dry, it is stable.
7. Collect all of the consecutive samples, and then return them to Outpatient services or 
your physician office. If you have been given a mailer, please return the collection cards by mail.

Stool Collection For Other Tests

You may be asked to collect a stool specimen for laboratory testing. You may collect this specimen in a clean container with a tightly fitting lid or in the collection kit provided by GPGDGC.

Patient Instructions

NOTE: Prior to collecting a stool specimen, avoid consuming the following:
1. Antacids
2. Antidiarrheal Medications 3. Oily Laxatives
4. Barium or Bismuth

These products can interfere with Test Results

Specimen Collection

1. Collect stool specimen into a clean, dry, disposable container.
(Cut off milk carton, cottage cheese or ice cream container, plastic bag, etc)

Do not allow urine or toilet water in container with stool

Note: If you have not been provided with special containers for returning your specimen, you may collect the specimen in a clean container with a tight fitting lid. Label the container with the patient’s name and the date of collection and return to GPGDCDC lab as soon as possible.

Add stool to empty vial. A normalamount would be the size of a one peso coin.

On container,  write your name, date, and time of collection.

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